Pang et al. 2020 Azaphilones biosynthesis complements the defence mechanism of Trichoderma guizhouense against oxidative stress, Environmental Microbiology

Guan et al., 2020 EM

Pang, G., Sun, T., Yu, Z., Yuan, T., Liu, W., Zhu, H., Gao, Q., Yang, D., Kubicek, C.P., Zhang, J., Shen, Q. (2020) Azaphilones biosynthesis complements the defence mechanism of Trichoderma guizhouense against oxidative stress, https://doi.org/10.1111/1462-2920.15246

Filamentous fungi are known as producers of a large array of diverse secondary metabolites (SMs) that aid in securing their environmental niche. Here, we demonstrated that the SMs have an additional role in fungal defense against other fungi: Trichoderma guizhouense, a mycoparasite, is able to antagonize Fusarium oxysporum f. sp. cubense race 4 (Foc4) by forming aerial hyphae that kill the host with hydrogen peroxide. At the same time, a gene cluster comprising two polyketide synthases is strongly expressed. Using functional genetics, we characterized this cluster and identified its products as azaphilones (termed as trigazaphilones). The trigazaphilones were found lacking in antifungal toxicity but exhibited high radical scavenging activities. The antioxidant property of trigazaphilones was in vivo functional under various tested conditions of oxidative stress. Thus, we conclude that the biosynthesis of trigazaphilones serves as a complementary antioxidant mechanism and defends T. guizhouense against the hydrogen peroxide that it produces to combat other fungi like Foc4.

Le Liu

Liu-Le-

Secretary

Office hours:

Monday to Friday 8:30 – 12:00 and 14:00 – 17:30

Phone: +86 (0)25 84396890

Fax: +86 (0)25 84396890

Email: 691279904@qq.com

About me:

I obtained my master’s degree from Nanjing Agricultural University in 2011. My master thesis research was focused on analyzing and identifying the mycorrhizal fungi associated with pine trees. After that, I had a two-years training in the molecular ecology of ectomycorrhizal fungi at the University of Tokyo. During that time, I also served as an instructor for international students in Japan and taught the Chinese language. In Nanjing, I worked as a high school English teacher. After the maternity leave, I joined FungiG in July 2019.