Irina S. Druzhinina, Ph. D.

Irina S. Druzhinina

Nanjing Agricultural University, College of Resources and Environmental Sciences

Weigang NO. 1, Nanjing, P.R. China, 210095 Tel +86 15 996344211

FungiG Fungal Genomics Group

Website:             www.FungiG.org

Email:                   Irina.druzhinina@njau.edu.cn

ORCID:                   0000-0003-2821-5268

Research Gate: https://www.researchgate.net/profile/Irina-Druzhinina

Google Scholar: https://scholar.google.com/citations?user=TRnrszkAAAAJ&hl=ru&oi=ao

Professor (Full), Fungal Genomics Laboratory (FungiG), Leader

Editor, Applied and Environmental Microbiology (AEM), ASM

Head of the TU Wien Collection of Industrial Microorganisms, Vienna, Austria

Associate Editor, Mycoasia

Editorial Board Member, Fungal Biology and Biotechnology, BMC

Head of the International Committee of Trichoderma Taxonomy (ICTT)

Coordinator of Trichoderma whole genus genomics project, JGI DOE, Berkeley, USA

Gao et al. 2020. The evolutionary and functional paradox of cerato-platanins in the mycoparasitic fungi. Applied and Environmental Microbiology

Cerato-platanins in hyphosphere

Gao, R., Ding, M., Jiang, S., Zhao, Z., Chenthamara, K., Shen, Q. Cai, F., Druzhinina I.S. 2020. The evolutionary and functional paradox of cerato-platanins in the mycoparasitic fungi. Applied and Environmental Microbiology 86:e00696-20

Cerato-platanins (CPs) form a family of fungal small secreted cysteine-rich proteins (SSCPs) and are of particular interest not only because of their surface activity but also their abundant secretion by fungi. We performed an evolutionary analysis of 283 CPs from 157 fungal genomes with the focus on the environmental opportunistic plant-beneficial and mycoparasitic fungus Trichoderma Our results revealed a long evolutionary history of CPs in Dikarya fungi that have undergone several events of lateral gene transfer and gene duplication. Three genes were maintained in the core genome of Trichoderma, while some species have up to four CP-encoding genes. All Trichoderma CPs evolve under stabilizing natural selection pressure. The functional genomic analysis of CPs in Trichoderma guizhouense and Trichoderma harzianum revealed that only epl1 is active at all stages of development but that it plays a minor role in interactions with other fungi and bacteria. The deletion of this gene results in increased colonization of tomato roots by Trichoderma spp. Similarly, biochemical tests of EPL1 heterologously produced by Pichia pastoris support the claims described above. Based on the results obtained, we conclude that the function of CPs is probably linked to their surfactant properties and the ability to modify the hyphosphere of submerged mycelia and, thus, facilitate the nutritional versatility of fungi. The effector-like functions do not sufficiently describe the diversity and evolution of these proteins in fungi, as they are also maintained, duplicated, or laterally transferred in the genomes of nonherbivore fungi.

Renwei Gao

FungiG Renwei Gao

I am a FungiG Ph.D. student interested in the genetic engineering and functional genetics of fungi. I feel fascinated by how wonderful the eukaryotic cell is. Although it is the result of evolution, it follows a particular rule as if it is arranged. My primary research is focused on the surface-active small secreted cysteine-rich proteins (saSSCPs) in Trichoderma, such as cerato-platanins and HFBs. 

My recent publications:

Gao, R., Ding, M., Jiang, S., Zhao, Z., Chenthamara, K., Shen, Q. Cai, F., Druzhinina I.S. 2020. The evolutionary and functional paradox of cerato-platanins in the mycoparasitic fungi. Applied and Environmental Microbiology 86:e00696-20

Cai, F., Gao, R., Zhao, Z., Ding, M., Jiang, S., Yagtu, C., Zhu, H., Zhang, J., Ebner, T., Mayrhofer-Reinhartshuber, M., Kainz, P., Chenthamara, K., Bayram-Akcapinar, G., Shen, Q., and Druzhinina, I. S. 2020 Evolutionary compromises in fungal fitness: hydrophobins hinder the adverse dispersal of spores and challenge their survival, The ISME J 14, 2610–2624 (2020). https://doi.org/10.1038/s41396-020-0709-0

Ding, M., Chen, W., Gao, R., Jiang, S., Zhao, Z., Cai, F., and Druzhinina, I. S. Emerging salt marshes as a source of Trichoderma arenerea sp. nov. and other fungal bioeffectors for biosaline agriculture, Journal of Applied Microbiology, doi: 10.1111/jam.14751.

Jiang S-Q, Yu Y-N, Gao R-W, Wang H, Zhang J, Li R, Long X-H, Shen Q-R, Chen W, Cai F: High-throughput absolute quantification sequencing reveals the effect of different fertilizer applications on bacterial community in a tomato cultivated coastal saline soil. Science of The Total Environment 2019, 687:601-609.